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A 16S rRNA gene fragment comprising the V3 and V4 hypervariable regions (16S sense 5-TACGGRAGGCAGCAG-3 and antisense 5-CTACCNGGGTATCTAAT-3) was amplified using an optimized and standardized 16S-amplicon-library preparation protocol (Metabiote, GenoScreen, Lille, France)

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When youre ready, they can help you build a stop smoking plan that works for you. For example, Quitline counsellors can give you advice about taking a break from alcohol which can be extremely helpful in the first few weeks of trying to quit, said Dr White

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